Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IF | See 4 publications below |
Product Information
25843-1-AP targets RAB11FIP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RAB11FIP3 fusion protein Ag22937 相同性解析による交差性が予測される生物種 |
| Full Name | RAB11 family interacting protein 3 (class II) |
| Calculated molecular weight | 756 aa, 82 kDa |
| Observed molecular weight | 82 kDa |
| GenBank accession number | BC051360 |
| Gene Symbol | RAB11FIP3 |
| Gene ID (NCBI) | 9727 |
| RRID | AB_2880264 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75154 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB11FIP3 antibody 25843-1-AP | Download protocol |
| IHC protocol for RAB11FIP3 antibody 25843-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Sci Rab11FIP1 maintains Rab35 at the intercellular bridge to promote actin removal and abscission. | ||
J Cell Sci Rab11FIP1 maintains Rab35 at the intercellular bridge to promote actin removal and abscission. | ||