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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB11FIP5 Polyclonal antibody (14594-1-AP)

    WB analysis using 14594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using RAB11FIP5 Polyclonal antibody (14594-1-AP)

    WB analysis of mouse kidney using 14594-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RAB11FIP5 Polyclonal antibody (14594-1-AP)

    WB analysis using 14594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RAB11FIP5 Polyclonal antibody (14594-1-AP)

    WB analysis using 14594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using RAB11FIP5 Polyclonal antibody (14594-1-AP)

    WB analysis of mouse lung using 14594-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RAB11FIP5 Polyclonal antibody (14594-1-AP)

    IHC staining of human cervical cancer using 14594-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RAB11FIP5 Polyclonal antibody (14594-1-AP)

    IHC staining of human cervical cancer using 14594-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RAB11FIP5 Polyclonal antibody (14594-1-AP)

    IHC staining of human stomach cancer using 14594-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RAB11FIP5 Polyclonal antibody (14594-1-AP)

    IHC staining of human stomach cancer using 14594-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using RAB11FIP5 Polyclonal antibody (14594-1-AP)

    IHC staining of mouse colon using 14594-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using RAB11FIP5 Polyclonal antibody (14594-1-AP)

    IHC staining of mouse colon using 14594-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RAB11FIP5 Polyclonal antibody (14594-1-AP)

    IHC staining of mouse kidney using 14594-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RAB11FIP5 Polyclonal antibody (14594-1-AP)

    IHC staining of mouse kidney using 14594-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using RAB11FIP5 Polyclonal antibody (14594-1-AP)

    IHC staining of rat colon using 14594-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using RAB11FIP5 Polyclonal antibody (14594-1-AP)

    IHC staining of rat colon using 14594-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using RAB11FIP5 Polyclonal antibody (14594-1-AP)

    IHC staining of rat kidney using 14594-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using RAB11FIP5 Polyclonal antibody (14594-1-AP)

    IHC staining of rat kidney using 14594-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

RAB11FIP5 Polyclonal antibody

RAB11FIP5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14594-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

GAF1, Gaf-1, Gamma-SNAP-associated factor 1, Rab11 family-interacting protein 5, Rab11-interacting protein Rip11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, mouse kidney tissue, mouse lung tissue, HeLa cells, mouse heart tissue, HEK-293T cells, rat heart tissue
Positive IHC detected inhuman cervical cancer tissue, mouse colon tissue, mouse kidney tissue, rat colon tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14594-1-AP targets RAB11FIP5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB11FIP5 fusion protein Ag6128 相同性解析による交差性が予測される生物種
Full Name RAB11 family interacting protein 5 (class I)
Calculated molecular weight 70 kDa
Observed molecular weight70 kDa
GenBank accession numberBC035013
Gene Symbol RAB11FIP5
Gene ID (NCBI) 26056
RRIDAB_10860101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXF6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab11 family-interacting protein 5 (Rab11fip5) is an adaptor protein that binds to the small GTPase Rab11, which has an important function in endosome recycling and trafficking of cellular proteins to the plasma membrane. Rab11fip5 is involved in many cellular processes, such as cytoskeleton rearrangement, iron uptake and exocytosis in neuroendocrine cells, and is also known as a candidate gene for autism-spectrum disorder.

Protocols

Product Specific Protocols
WB protocol for RAB11FIP5 antibody 14594-1-APDownload protocol
IHC protocol for RAB11FIP5 antibody 14594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nat Commun

Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules.

Authors - Kai He
  • KD Validated
View Article