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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB12 Polyclonal antibody

RAB12 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 29927-1-AP

Synonyms

Ras-related protein Rab-12, Ras related protein Rab 12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB12 Polyclonal antibody (29927-1-AP)

    WB analysis using 29927-1-AP

    Human brain tissue was subjected to SDS PAGE followed by western blot with 29927-1-AP (RAB12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RAB12 Polyclonal antibody (29927-1-AP)

    WB analysis using 29927-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29927-1-AP (RAB12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using RAB12 Polyclonal antibody (29927-1-AP)

    IP experiment of mouse brain using 29927-1-AP

    IP result of anti-RAB12 (IP:29927-1-AP, 4ug; Detection:29927-1-AP 1:1500) with mouse brain tissue lysate 2100 ug.

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Tested Applications

Positive WB detected inhuman brain tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29927-1-AP targets RAB12 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB12 fusion protein Ag31627 相同性解析による交差性が予測される生物種
Full Name RAB12, member RAS oncogene family
Calculated molecular weight27KD
Observed molecular weight 27 kDa
GenBank accession numberNM_001025300
Gene symbol RAB12
Gene ID (NCBI) 201475
RRIDAB_3669700
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab12, a previously uncharacterized Rab isoform, is mainly localized at TfR-positive recycling endosomes and is specifically involved in TfR degradation. Rab12 is a regulator of LRRK2 and its activation by damaged lysosomes.

Protocols

Product Specific Protocols
WB protocol for RAB12 antibody 29927-1-APDownload protocol
IP protocol for RAB12 antibody 29927-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols