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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB13 Polyclonal antibody, PBS Only

RAB13 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11718-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB13 Polyclonal antibody (11718-1-AP)

    WB analysis using 11718-1-AP (same clone as 11718-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11718-1-AP (RAB13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11718-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RAB13 Polyclonal antibody (11718-1-AP)

    WB analysis using 11718-1-AP (same clone as 11718-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11718-1-AP (RAB13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11718-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB13 Polyclonal antibody (11718-1-AP)

    IF Staining of HeLa using 11718-1-AP (same clone as 11718-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (11718-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 11718-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using RAB13 Polyclonal antibody (11718-1-AP)

    FC experiment of K-562 using 11718-1-AP (same clone as 11718-1-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.4 ug RAB13 Polyclonal antibody (11718-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11718-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11718-1-PBS targets RAB13 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB13 fusion protein Ag2292 相同性解析による交差性が予測される生物種
Full Name RAB13, member RAS oncogene family
Calculated molecular weight 203 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC000799
Gene symbol RAB13
Gene ID (NCBI) 5872
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).