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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB13 Polyclonal antibody

RAB13 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 31416-1-AP

Synonyms

GIG4, Ras-related protein Rab-13, Ras related protein Rab 13, Cell growth-inhibiting gene 4 protein, Cell growth inhibiting gene 4 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB13 Polyclonal antibody (31416-1-AP)

    WB analysis using 31416-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31416-1-AP (RAB13 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RAB13 Polyclonal antibody (31416-1-AP)

    IHC staining of mouse testis using 31416-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31416-1-AP (RAB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using RAB13 Polyclonal antibody (31416-1-AP)

    IHC staining of mouse stomach using 31416-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31416-1-AP (RAB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of K-562 cells using RAB13 Polyclonal antibody (31416-1-AP)

    FC experiment of K-562 using 31416-1-AP

    1x10^6 K-562 cells were intracellularly stained with 0.8 ug RAB13 Polyclonal antibody (31416-1-AP) and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, MCF-7 cells
Positive IHC detected inmouse testis tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31416-1-AP targets RAB13 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB13 fusion protein Ag34898 相同性解析による交差性が予測される生物種
Full Name RAB13, member RAS oncogene family
Calculated molecular weight 203 aa, 23 kDa
Observed molecular weight 20-23 kDa
GenBank accession numberBC000799
Gene symbol RAB13
Gene ID (NCBI) 5872
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).

Protocols

Product Specific Protocols
WB protocol for RAB13 antibody 31416-1-APDownload protocol
IHC protocol for RAB13 antibody 31416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols