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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB13 Recombinant antibody, PBS Only (Capture)

RAB13 Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240216D10

Cat no : 83336-2-PBS

Synonyms

GIG4, Ras-related protein Rab-13, Ras related protein Rab 13, Cell growth-inhibiting gene 4 protein, Cell growth inhibiting gene 4 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB13 Recombinant antibody (83336-2-RR)

    WB analysis using 83336-2-RR (same clone as 83336-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83336-2-RR (RAB13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB13 Recombinant antibody (83336-2-RR)

    IF Staining of HeLa using 83336-2-RR (same clone as 83336-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (83336-2-RR, Clone: 240216D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB13 Recombinant antibody (83336-2-RR)

    IF Staining of HeLa using 83336-2-RR (same clone as 83336-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (83336-2-RR, Clone: 240216D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using RAB13 Recombinant antibody (83336-2-RR)

    FC experiment of K-562 using 83336-2-RR (same clone as 83336-2-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug RAB13 Recombinant antibody (83336-2-RR, Clone:240216D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00354-1

    Cytometric bead array standard curve of MP00354-1

    Cytometric bead array standard curve of MP00354-1, RAB13 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83336-2-PBS. Detection antibody: 83336-1-PBS. Standard: Ag34898. Range: 0.156-20 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83336-2-PBS targets RAB13 as part of a matched antibody pair:

MP00354-1: 83336-2-PBS capture and 83336-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAB13 fusion protein Ag34898 相同性解析による交差性が予測される生物種
Full Name RAB13, member RAS oncogene family
Calculated molecular weight 203 aa, 23 kDa
Observed molecular weight23 kDa
GenBank accession numberBC000799
Gene symbol RAB13
Gene ID (NCBI) 5872
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).