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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB13 Recombinant antibody

RAB13 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240216D10

Cat no : 83336-2-RR

Synonyms

GIG4, Ras-related protein Rab-13, Ras related protein Rab 13, Cell growth-inhibiting gene 4 protein, Cell growth inhibiting gene 4 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB13 Recombinant antibody (83336-2-RR)

    WB analysis using 83336-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83336-2-RR (RAB13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB13 Recombinant antibody (83336-2-RR)

    IF Staining of HeLa using 83336-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (83336-2-RR, Clone: 240216D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB13 Recombinant antibody (83336-2-RR)

    IF Staining of HeLa using 83336-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (83336-2-RR, Clone: 240216D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of K-562 cells using RAB13 Recombinant antibody (83336-2-RR)

    FC experiment of K-562 using 83336-2-RR

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug RAB13 Recombinant antibody (83336-2-RR, Clone:240216D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, L02 cells, SW480 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83336-2-RR is RAB13 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAB13 fusion protein Ag34898 相同性解析による交差性が予測される生物種
Full Name RAB13, member RAS oncogene family
Calculated molecular weight 203 aa, 23 kDa
Observed molecular weight23 kDa
GenBank accession numberBC000799
Gene symbol RAB13
Gene ID (NCBI) 5872
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).

Protocols

Product Specific Protocols
WB protocol for RAB13 antibody 83336-2-RRDownload protocol
IF protocol for RAB13 antibody 83336-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols