Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, Jurkat cells, human testis tissue, pig brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:175-1:700 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
67659-1-Ig targets RAB18 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Reactivity | Human, mouse, rat, pig |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RAB18 fusion protein Ag16669 相同性解析による交差性が予測される生物種 |
| Full Name | RAB18, member RAS oncogene family |
| Calculated molecular weight | 206 aa, 23 kDa |
| GenBank accession number | BC015014 |
| Gene Symbol | RAB18 |
| Gene ID (NCBI) | 22931 |
| RRID | AB_2882856 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NP72 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB18 antibody 67659-1-Ig | Download protocol |
| IHC protocol for RAB18 antibody 67659-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Gastroenterol Hepatol Effect of Rab18 on liver injury and lipid accumulation by regulating perilipin 2 and peroxisome proliferator-activated receptor gamma in non-alcoholic fatty liver disease |