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KD/KO Validated

RAB1B Polyclonal antibody

Name: RAB1B Polyclonal antibody
Brand: Proteintech
SKU: 17824-1-AP

RAB1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17824-1-AP

Synonyms

Ras-related protein Rab-1B, Ras related protein Rab 1B, EC:3.6.5.2

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 17824-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17824-1-AP (RAB1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HAP1 using 17824-1-AP
HAP1 (WT and RAB1A and RAB1B KO) lysates prepared with RIPA buffer, 30 μg of protein loaded. 17824-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
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HAP1 (WT and RAB1A and RAB1B KO) lysates prepared with RIPA buffer, 30 μg of protein loaded. 17824-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
WB analysis of HeLa using 17824-1-AP
WB result of RAB1B antibody (17824-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB1B transfected HeLa cells.
IHC staining of human heart using 17824-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17824-1-AP (RAB1B Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human heart using 17824-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17824-1-AP (RAB1B Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human ovary tumor using 17824-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17824-1-AP (RAB1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 17824-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17824-1-AP (RAB1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 17824-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17824-1-AP (RAB1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of NIH/3T3 using 17824-1-AP
Immunofluorescent analysis of NIH/3T3 cells using 17824-1-AP (RAB1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 17824-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAB1B antibody (17824-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 17824-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A375 cells, HeLa cells, HAP1, A431 cells, NIH/3T3 cells
Positive IHC detected in	human heart tissue, mouse heart tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 12 publications below
IHC	See 1 publications below
IF	See 4 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

17824-1-AP targets RAB1B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RAB1B fusion protein Ag12197 相同性解析による交差性が予測される生物種
Full Name	RAB1B, member RAS oncogene family
Calculated molecular weight	201 aa, 22 kDa
Observed molecular weight	22-25 kDa
GenBank accession number	BC071169
Gene symbol	RAB1B
Gene ID (NCBI)	81876
RRID	AB_2237881
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ras-related protein Rab-1B (RAB1B) belongs to the small GTPase superfamily and Rab family. Rab1b, a GTPase regulating secretory transport, was recently associated with targeting proteins to LDs in a Drosophila RNAi screen. Rab1b recruits lipid-synthesizing enzymes, facilitating Lipid droplets (LDs) growth. The small GTPase Rab1b interacts with diacylglycerol acyltransferase 2 to facilitate its targeting to the surface of lipid droplets (PMID: 38809969). Rab1b, an extensively studied and established master regulator of ER-to-Golgi transport. The mechanism of Rab1b's function is to promote ER to LD surface targeting of those triglyceride-synthesizing enzymes that can associate with the unique lipid monolayer comprising the LD surface (PMID: 38809969). Rab1b is a regulatory protein involved in both COPI and COPII transport (PMID: 17429068).

Protocols

Product Specific Protocols
WB protocol for RAB1B antibody 17824-1-AP	Download protocol
IHC protocol for RAB1B antibody 17824-1-AP	Download protocol
IF protocol for RAB1B antibody 17824-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Cell Rab1A Is an mTORC1 Activator and a Colorectal Oncogene. Authors - Janice D Thomas View Article
human	WB,IF	Nat Cell Biol A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion Authors - Yuxin Sun KD Validated View Article
human	WB	Nat Cell Biol A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens. Authors - Reiling Jan H JH View Article
human	IF	Dev Cell SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport. Authors - Rui Yan View Article
human	IHC	Aging (Albany NY) Overexpression of Rab1B and MMP9 predicts poor survival and good response to chemotherapy in patients with colorectal cancer. Authors - Xian-Zi Yang View Article
rat	WB	Front Pharmacol Fuyuan Xingnao Decoction Promotes Angiogenesis Through the Rab1/AT1R Pathway in Diabetes Mellitus Complicated With Cerebral Infarction. Authors - Dong Deng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB1B Polyclonal antibody

RAB1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 17824-1-AP

WB analysis of HAP1 using 17824-1-AP

WB analysis of HeLa using 17824-1-AP

IHC staining of human heart using 17824-1-AP

IHC staining of human heart using 17824-1-AP

IHC staining of human ovary tumor using 17824-1-AP

IHC staining of human ovary tumor using 17824-1-AP

IHC staining of mouse heart using 17824-1-AP

IF Staining of NIH/3T3 using 17824-1-AP

IF Staining of HeLa using 17824-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Rab1A Is an mTORC1 Activator and a Colorectal Oncogene.

A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion

A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.

SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport.

Overexpression of Rab1B and MMP9 predicts poor survival and good response to chemotherapy in patients with colorectal cancer.

Fuyuan Xingnao Decoction Promotes Angiogenesis Through the Rab1/AT1R Pathway in Diabetes Mellitus Complicated With Cerebral Infarction.