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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB21 Polyclonal antibody (31303-1-AP)

    WB analysis using 31303-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31303-1-AP (RAB21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RAB21 Polyclonal antibody (31303-1-AP)

    WB analysis using 31303-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31303-1-AP (RAB21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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RAB21 Polyclonal antibody

RAB21 Polyclonal Antibody for WB, ELISA
Cat No. 31303-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Ras related protein Rab 21, KIAA0118

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMDA-MB-231 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31303-1-AP targets RAB21 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB21 fusion protein Ag35291 相同性解析による交差性が予測される生物種
Full Name RAB21, member RAS oncogene family
Calculated molecular weight24 kDa
Observed molecular weight24 kDa
GenBank accession numberNM_014999
Gene Symbol RAB21
Gene ID (NCBI) 23011
RRIDAB_3669940
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9UL25
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Small GTPase Rab21 is a ubiquitously expressed and has recently been shown to associate with the α tails of several integrins via the shared conserved membrane proximal sequence, thus regulating cell adhesion and migration via controlling the endo/exocytic trafficking of most integrin heterodimers (PMID: 18804435). Rab21 is required for the pruning of the immature neurites during the multipolar-to-bipolar transition (PMID: 36683567). Knock down of Rab21 impairs integrin-mediated cell adhesion and motility, whereas its overexpression stimulates cell migration and cancer cell adhesion to collagen and human bone (PMID: 16754960).

Protocols

Product Specific Protocols
WB protocol for RAB21 antibody 31303-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols