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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using Rab23 Monoclonal antibody (60056-1-Ig)

    WB analysis of human brain using 60056-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 60056-1-Ig (RAB23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Rab23 Monoclonal antibody (60056-1-Ig)

    WB analysis of HeLa using 60056-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60056-1-Ig (RAB23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Rab23 Monoclonal antibody (60056-1-Ig)

    WB analysis of human brain using 60056-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 60056-1-Ig (RAB23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  • Featured Product
  • KD/KO Validated

Rab23 Monoclonal antibody

Rab23 Monoclonal Antibody for WB, ELISA
Cat No. 60056-1-Ig
Clone No.3E12B6
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman brain tissue, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60056-1-Ig targets Rab23 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Rab23 fusion protein Ag1588 相同性解析による交差性が予測される生物種
Full Name RAB23, member RAS oncogene family
Calculated molecular weight 237 aa, 27 kDa
Observed molecular weight 27-30 kDa
GenBank accession numberBC015021
Gene Symbol RAB23
Gene ID (NCBI) 51715
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9ULC3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab23 is a novel member of large Rab small GTPase family. It plays important roles during embryonic development. The brain-enriched Rab23 is the only Rab protein that is known to have a distinct function during central nervous system development, playing an essential role as a negative regulator of the Sonic Hedgehog (Shh) pathway. RAB23 has recently been identified in mesangial cells (MCs), and could serve as a biomarker that indicates the severity of FSGS ( focal segmental glomerulosclerosis). Mutations in RAB23 may result in ACPS2 ( acrocephalopolysyndactyly type 2).

Publications

SpeciesApplicationTitle
human,mouseWB

Curr Biol

Planar Cell Polarity Effector Proteins Inturned and Fuzzy Form a Rab23 GEF Complex.

Authors - Andreas Gerondopoulos
  • KD Validated
View Article