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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB24 Polyclonal antibody (11445-1-AP)

    WB analysis using 11445-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RAB24 Polyclonal antibody (11445-1-AP)

    WB analysis using 11445-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RAB24 Polyclonal antibody (11445-1-AP)

    WB analysis of HEK-293 using 11445-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using RAB24 Polyclonal antibody (11445-1-AP)

    WB analysis of human skeletal muscle using 11445-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using RAB24 Polyclonal antibody (11445-1-AP)

    IP experiment of mouse brain using 11445-1-AP

    IP result of anti-RAB24 (IP:11445-1-AP, 4ug; Detection:11445-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RAB24 Polyclonal antibody (11445-1-AP)

    IHC staining of human gliomas using 11445-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11445-1-AP (RAB24 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RAB24 Polyclonal antibody (11445-1-AP)

    IHC staining of human gliomas using 11445-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11445-1-AP (RAB24 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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RAB24 Polyclonal antibody

RAB24 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11445-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Synonyms

RAB24, Ras related protein Rab 24

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, human skeletal muscle tissue, fetal human brain tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11445-1-AP targets RAB24 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB24 fusion protein Ag1995 相同性解析による交差性が予測される生物種
Full Name RAB24, member RAS oncogene family
Calculated molecular weight 203 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC021263
Gene Symbol RAB24
Gene ID (NCBI) 53917
RRIDAB_2269046
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969Q5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB24 is a small GTPase of the Rab subfamily of Ras-related proteins that regulate intracellular protein trafficking. Unlike other Rab family members, RAB24 does not interact with GDP dissociation inhibitors (GDIs), including ARHGDIA and ARHGDIB. It Interacts with ZFYVE20. Rab24 modulates several mitotic events, including chromosome segregation and cytokinesis, perhaps through the interaction with microtubules.

Protocols

Product Specific Protocols
WB protocol for RAB24 antibody 11445-1-APDownload protocol
IHC protocol for RAB24 antibody 11445-1-APDownload protocol
IP protocol for RAB24 antibody 11445-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
canineIHC

PLoS Genet

Canine Hereditary Ataxia in Old English Sheepdogs and Gordon Setters Is Associated with a Defect in the Autophagy Gene Encoding RAB24.

Authors - Caryline Agler
View Article
humanWB

Dose Response

Genome-Wide Identification of Autophagy Prognostic Signature in Pancreatic Cancer.

Authors - Jianfa Yu
View Article