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Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using RAB25 Polyclonal antibody (13189-1-AP)

    WB analysis of HT-29 using 13189-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 13189-1-AP (RAB25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of T-47D cells using RAB25 Polyclonal antibody (13189-1-AP)

    WB analysis of T-47D using 13189-1-AP

    T-47D cells were subjected to SDS PAGE followed by western blot with 13189-1-AP (RAB25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Caco-2 cells using RAB25 Polyclonal antibody (13189-1-AP)

    WB analysis of Caco-2 using 13189-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 13189-1-AP (RAB25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using RAB25 Polyclonal antibody (13189-1-AP)

    IHC staining of human renal cell carcinoma using 13189-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using RAB25 Polyclonal antibody (13189-1-AP)

    IHC staining of human renal cell carcinoma using 13189-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RAB25 Polyclonal antibody (13189-1-AP)

    IHC staining of human kidney using 13189-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RAB25 Polyclonal antibody (13189-1-AP)

    IHC staining of human kidney using 13189-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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RAB25 Polyclonal antibody

RAB25 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13189-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CATX 8, CATX8, RAB25, Ras related protein Rab 25

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-29 cells, Caco-2 cells, T-47D cells
Positive IHC detected inhuman renal cell carcinoma tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

13189-1-AP targets RAB25 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB25 fusion protein Ag3864 相同性解析による交差性が予測される生物種
Full Name RAB25, member RAS oncogene family
Calculated molecular weight 213 aa, 23 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC033322
Gene Symbol RAB25
Gene ID (NCBI) 57111
RRIDAB_2877921
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP57735
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAB25 antibody 13189-1-APDownload protocol
IHC protocol for RAB25 antibody 13189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Transl Cancer Res

Rab25 acts as an oncogene and participates in the regulation of aerobic glycolysis via PKM2 in gastric adenocarcinoma

Authors - Tianyu Zheng
View Article