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- KD/KO Validated
RAB27A Polyclonal antibody
RAB27A Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (4)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 17817-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, human liver tissue, HeLa cells, L02 cells, K-562 cells, K562, Jurkat cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human malignant melanoma tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 13 publications below |
| WB | See 54 publications below |
| IHC | See 4 publications below |
| IF | See 7 publications below |
Product Information
17817-1-AP targets RAB27A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat, pig, canine, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RAB27A fusion protein Ag12136 相同性解析による交差性が予測される生物種 |
| Full Name | RAB27A, member RAS oncogene family |
| Calculated molecular weight | 221 aa, 25 kDa |
| Observed molecular weight | 25-27 kDa |
| GenBank accession number | BC107680 |
| Gene symbol | RAB27A |
| Gene ID (NCBI) | 5873 |
| RRID | AB_2176728 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB27A antibody 17817-1-AP | Download protocol |
| IHC protocol for RAB27A antibody 17817-1-AP | Download protocol |
| IP protocol for RAB27A antibody 17817-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Immunol Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity. | ||
J Allergy Clin Immunol A RAB27A 5' untranslated region structural variant associated with late-onset hemophagocytic lymphohistiocytosis and normal pigmentation. | ||
Nat Commun Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.
| ||
Blood Extracellular vesicles released by CD40/IL-4 stimulated chronic lymphocytic leukemia cells confer altered functional properties to CD4+ T cells. |
