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  • Featured Product
  • KD/KO Validated

RAB27A Polyclonal antibody, PBS Only

RAB27A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17817-1-PBS

Synonyms

Rab-27, RAB27, Rab 27, GTP-binding protein Ram, GTP binding protein Ram

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB27A Polyclonal antibody (17817-1-AP)

    WB analysis using 17817-1-AP (same clone as 17817-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K562 using RAB27A Polyclonal antibody (17817-1-AP)

    WB analysis of K562 using 17817-1-AP (same clone as 17817-1-PBS)

    WB result of RAB27A antibody (17817-1-AP, 1:3000) with si-Control and si-RAB7B transfected K562 cells. This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using RAB27A Polyclonal antibody (17817-1-AP)

    WB analysis of L02 using 17817-1-AP (same clone as 17817-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using RAB27A Polyclonal antibody (17817-1-AP)

    WB analysis of K-562 using 17817-1-AP (same clone as 17817-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using RAB27A Polyclonal antibody (17817-1-AP)

    WB analysis of human liver using 17817-1-AP (same clone as 17817-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RAB27A Polyclonal antibody (17817-1-AP)

    WB analysis of HeLa using 17817-1-AP (same clone as 17817-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using RAB27A Polyclonal antibody (17817-1-AP)

    IP experiment of K-562 using 17817-1-AP (same clone as 17817-1-PBS)

    IP result of anti-RAB27A (IP:17817-1-AP, 4ug; Detection:17817-1-AP 1:1000) with K-562 cells lysate 2760 ug. This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using RAB27A Polyclonal antibody (17817-1-AP)

    IHC staining of human malignant melanoma using 17817-1-AP (same clone as 17817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using RAB27A Polyclonal antibody (17817-1-AP)

    IHC staining of human malignant melanoma using 17817-1-AP (same clone as 17817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RAB27A Polyclonal antibody (17817-1-AP)

    IHC staining of human stomach using 17817-1-AP (same clone as 17817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RAB27A Polyclonal antibody (17817-1-AP)

    IHC staining of human stomach using 17817-1-AP (same clone as 17817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17817-1-PBS targets RAB27A in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB27A fusion protein Ag12136 相同性解析による交差性が予測される生物種
Full Name RAB27A, member RAS oncogene family
Calculated molecular weight 221 aa, 25 kDa
Observed molecular weight 25-27 kDa
GenBank accession numberBC107680
Gene symbol RAB27A
Gene ID (NCBI) 5873
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.