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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB27A Monoclonal antibody (66058-1-Ig)

    WB analysis using 66058-1-Ig (same clone as 66058-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66058-1-Ig (RAB27A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K562 using RAB27A Monoclonal antibody (66058-1-Ig)

    WB analysis of K562 using 66058-1-Ig (same clone as 66058-1-PBS)

    WB result of RAB27A antibody (66058-1-Ig, 1:5000) with si-Control and si-RAB27A transfected K562 cells. This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using RAB27A Monoclonal antibody (66058-1-Ig)

    WB analysis of K-562 using 66058-1-Ig (same clone as 66058-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66058-1-Ig (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using RAB27A Monoclonal antibody (66058-1-Ig)

    IHC staining of human lymphoma using 66058-1-Ig (same clone as 66058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using RAB27A Monoclonal antibody (66058-1-Ig)

    IHC staining of human lymphoma using 66058-1-Ig (same clone as 66058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAB27A Monoclonal antibody (66058-1-Ig)

    IHC staining of human colon cancer using 66058-1-Ig (same clone as 66058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAB27A Monoclonal antibody (66058-1-Ig)

    IHC staining of human colon cancer using 66058-1-Ig (same clone as 66058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using RAB27A Monoclonal antibody (66058-1-Ig)

    IHC staining of human skeletal muscle using 66058-1-Ig (same clone as 66058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 66058-1-Ig(RAB27A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using RAB27A Monoclonal antibody (66058-1-Ig)

    IHC staining of human skeletal muscle using 66058-1-Ig (same clone as 66058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 66058-1-Ig(RAB27A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

RAB27A Monoclonal antibody, PBS Only

RAB27A Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66058-1-PBS
Clone No.1C4B8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66058-1-PBS targets RAB27A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAB27A fusion protein Ag12382 相同性解析による交差性が予測される生物種
Full Name RAB27A, member RAS oncogene family
Calculated molecular weight 221 aa, 25 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC107680
Gene Symbol RAB27A
Gene ID (NCBI) 5873
RRIDAB_11042596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51159
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.