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  • Featured Product
  • KD/KO Validated

RAB27A Polyclonal antibody

RAB27A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16868-1-AP

Synonyms

RAB27A-specific, Rab-27, RAB27, Rab 27, GTP-binding protein Ram

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB27A-specific Polyclonal antibody (16868-1-AP)

    WB analysis using 16868-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16868-1-AP (RAB27A-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K562 using RAB27A-specific Polyclonal antibody (16868-1-AP)

    WB analysis of K562 using 16868-1-AP

    WB result of RAB27A antibody (16868-1-AP, 1:1000) with si-Control and si-RAB27A transfected K562 cells.

  • Western Blot (WB) analysis of HL-60 cells using RAB27A-specific Polyclonal antibody (16868-1-AP)

    WB analysis of HL-60 using 16868-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 16868-1-AP (RAB27A-specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using RAB27A-specific Polyclonal antibody (16868-1-AP)

    IHC staining of human lymphoma using 16868-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using RAB27A-specific Polyclonal antibody (16868-1-AP)

    IHC staining of human lymphoma using 16868-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RAB27A-specific Polyclonal antibody (16868-1-AP)

    IHC staining of human liver cancer using 16868-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RAB27A-specific Polyclonal antibody (16868-1-AP)

    IHC staining of human liver cancer using 16868-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHL-60 cells, K562, PC-12 cells, U-87 MG cells
Positive IHC detected inhuman lymphoma tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16868-1-AP targets RAB27A in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name RAB27A, member RAS oncogene family
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberNM_004580
Gene symbol RAB27A
Gene ID (NCBI) 5873
RRIDAB_2176716
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.

Protocols

Product Specific Protocols
WB protocol for RAB27A antibody 16868-1-APDownload protocol
IHC protocol for RAB27A antibody 16868-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biochem Biophys Res Commun

Involvement of Rab11 in osteoblastic differentiation: Its up-regulation during the differentiation and by tensile stress

Authors - Lay Thant
View Article