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  • Featured Product
  • KD/KO Validated

RAB27B Polyclonal antibody

RAB27B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13412-1-AP

Synonyms

C25KG, Ras-related protein Rab-27B, Ras related protein Rab 27B, EC:3.6.5.2

Validation Data Gallery

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  • Western Blot (WB) analysis of rat spleen tissue using RAB27B Polyclonal antibody (13412-1-AP)

    WB analysis of rat spleen using 13412-1-AP

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using RAB27B Polyclonal antibody (13412-1-AP)

    WB analysis of A375 using 13412-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using RAB27B Polyclonal antibody (13412-1-AP)

    WB analysis of DU 145 using 13412-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using RAB27B Polyclonal antibody (13412-1-AP)

    WB analysis of PC-3 using 13412-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RAB27B Polyclonal antibody (13412-1-AP)

    WB analysis of MCF-7 using 13412-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat stomach tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of rat stomach using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of mouse stomach using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human pancreas cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of rat stomach using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human pancreas cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human oesophagus cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human oesophagus cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human colon cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human colon cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human breast cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human breast cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human breast cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human breast cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human colon cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human colon cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human testis using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human testis using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human prostate cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human prostate cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human liver cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human liver cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human prostate cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RAB27B Polyclonal antibody (13412-1-AP)

    IHC staining of human prostate cancer using 13412-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB27B Polyclonal antibody (13412-1-AP)

    IF Staining of HeLa using 13412-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13412-1-AP (RAB27B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U-87 MG cells using RAB27B Polyclonal antibody (13412-1-AP)

    IF Staining of U-87 MG using 13412-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using RAB27B antibody (13412-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inrat spleen tissue, A375 cells, DU 145 cells, MCF-7 cells, PC-3 cells
Positive IHC detected inrat stomach tissue, human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, human oesophagus cancer tissue, human pancreas cancer tissue, human prostate cancer tissue, human testis tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-87 MG cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13412-1-AP targets RAB27B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB27B fusion protein Ag4064 相同性解析による交差性が予測される生物種
Full Name RAB27B, member RAS oncogene family
Calculated molecular weight 218 aa, 25 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC027474
Gene symbol RAB27B
Gene ID (NCBI) 5874
RRIDAB_2176732
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.

Protocols

Product Specific Protocols
WB protocol for RAB27B antibody 13412-1-APDownload protocol
IHC protocol for RAB27B antibody 13412-1-APDownload protocol
IF protocol for RAB27B antibody 13412-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth.

Authors - Lin Zhang
View Article
humanWB, IHC

Cell

Exosome transfer from stromal to breast cancer cells regulates therapy resistance pathways.

Authors - Mirjam C Boelens
View Article
humanWB

Immunity

MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation.

Authors - Seongmin Yoon
View Article
humanWB

Adv Sci (Weinh)

Exosome Release Is Regulated by mTORC1.

Authors - Wenchong Zou
View Article
mouseIF

Nat Commun

Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.

Authors - I-Ling Lu
View Article
humanWB

Nat Commun

Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.

Authors - Akiko Takahashi
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Kacie (Verified Customer) (09-12-2022)

Antibody works well for both ICC and WB.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: M17 Cells