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  • Featured Product
  • KD/KO Validated

RAB27B Monoclonal antibody, PBS Only

RAB27B Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B9G9

Cat no : 66944-1-PBS

Synonyms

C25KG, Ras-related protein Rab-27B, Ras related protein Rab 27B, EC:3.6.5.2, 2B9G9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB27B Monoclonal antibody (66944-1-Ig)

    WB analysis using 66944-1-Ig (same clone as 66944-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66944-1-Ig (RAB27B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66944-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood platelets using RAB27B Monoclonal antibody (66944-1-Ig)

    WB analysis of human peripheral blood platelets using 66944-1-Ig (same clone as 66944-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66944-1-Ig (RAB27B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66944-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RAB27B Monoclonal antibody (66944-1-Ig)

    IHC staining of human lung cancer using 66944-1-Ig (same clone as 66944-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66944-1-Ig (RAB27B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66944-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RAB27B Monoclonal antibody (66944-1-Ig)

    IHC staining of human lung cancer using 66944-1-Ig (same clone as 66944-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66944-1-Ig (RAB27B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66944-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RAB27B Monoclonal antibody (66944-1-Ig)

    IF Staining of A431 using 66944-1-Ig (same clone as 66944-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RAB27B antibody (66944-1-Ig, Clone: 2B9G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66944-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using RAB27B Monoclonal antibody (66944-1-Ig)

    FC experiment of A431 using 66944-1-Ig (same clone as 66944-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RAB27B (66944-1-Ig, Clone:2B9G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66944-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66944-1-PBS targets RAB27B in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAB27B fusion protein Ag18756 相同性解析による交差性が予測される生物種
Full Name RAB27B, member RAS oncogene family
Calculated molecular weight 218 aa, 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC027474
Gene symbol RAB27B
Gene ID (NCBI) 5874
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.