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KD/KO Validated

RAB2 Polyclonal antibody

Name: RAB2 Polyclonal antibody
Brand: Proteintech
SKU: 15420-1-AP

RAB2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15420-1-AP

Synonyms

RAB2, RAB2A, Ras related protein Rab 2A

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of mouse brain using 15420-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 15420-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15420-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 15420-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15420-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15420-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 15420-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15420-1-AP (RAB2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 15420-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15420-1-AP (RAB2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human breast cancer using 15420-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 15420-1-AP (RAB2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 15420-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 15420-1-AP (RAB2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 15420-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 15420-1-AP (RAB2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 15420-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 15420-1-AP (RAB2 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 15420-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, HEK-293 cells, HeLa cells, mouse lung tissue, rat brain tissue
Positive IHC detected in	human brain tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 11 publications below
IF	See 2 publications below

Product Information

15420-1-AP targets RAB2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RAB2 fusion protein Ag7708 相同性解析による交差性が予測される生物種
Full Name	RAB2A, member RAS oncogene family
Calculated molecular weight	24 kDa
Observed molecular weight	24 kDa
GenBank accession number	BC008929
Gene symbol	RAB2A
Gene ID (NCBI)	5862
RRID	AB_2176874
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize both RAB2A and RAB2B.

Protocols

Product Specific Protocols
WB protocol for RAB2 antibody 15420-1-AP	Download protocol
IHC protocol for RAB2 antibody 15420-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy Authors - Shen Zhang View Article
human	WB	Cell Host Microbe A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication. Authors - Cheryl N Miller KD Validated View Article
human	WB	Nat Commun Arsenic targets Pin1 and cooperates with retinoic acid to inhibit cancer-driving pathways and tumor-initiating cells. Authors - Shingo Kozono View Article
		Proc Natl Acad Sci U S A Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion. Authors - Liang Chen View Article
human	WB	Cell Rep Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes Authors - Ariane Schleinitz View Article
		EMBO Rep RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs. Authors - Hiroaki Kajiho View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB2 Polyclonal antibody

RAB2 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of mouse brain using 15420-1-AP

WB analysis of rat brain using 15420-1-AP

WB analysis of HEK-293 using 15420-1-AP

WB analysis of mouse lung using 15420-1-AP

WB analysis of HEK-293 using 15420-1-AP

WB analysis of HeLa using 15420-1-AP

IHC staining of human brain using 15420-1-AP

IHC staining of human brain using 15420-1-AP

IHC staining of human breast cancer using 15420-1-AP

IHC staining of human breast cancer using 15420-1-AP

IHC staining of human breast cancer using 15420-1-AP

IHC staining of human breast cancer using 15420-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy

A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication.

Arsenic targets Pin1 and cooperates with retinoic acid to inhibit cancer-driving pathways and tumor-initiating cells.

Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.

Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes

RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs.