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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB2A Monoclonal antibody (67501-1-Ig)

    WB analysis using 67501-1-Ig (same clone as 67501-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67501-1-Ig (RAB2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67501-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RAB2A Monoclonal antibody (67501-1-Ig)

    IHC staining of mouse brain using 67501-1-Ig (same clone as 67501-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67501-1-Ig (RAB2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67501-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RAB2A Monoclonal antibody (67501-1-Ig)

    IHC staining of mouse brain using 67501-1-Ig (same clone as 67501-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67501-1-Ig (RAB2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67501-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using RAB2A Monoclonal antibody (67501-1-Ig)

    IF Staining of mouse brain using 67501-1-Ig (same clone as 67501-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67501-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using RAB2A Monoclonal antibody (67501-1-Ig)

    IF Staining of mouse brain using 67501-1-Ig (same clone as 67501-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67501-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RAB2A Monoclonal antibody (67501-1-Ig)

    IF Staining of U2OS using 67501-1-Ig (same clone as 67501-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67501-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB2A Monoclonal antibody (67501-1-Ig)

    IF Staining of HeLa using 67501-1-Ig (same clone as 67501-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67501-1-PBS in a different storage buffer formulation.

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RAB2A Monoclonal antibody, PBS Only

RAB2A Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 67501-1-PBS
Clone No.3E1B10

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

RAB2, Ras-related protein Rab-2A, Ras related protein Rab 2A, EC:3.6.5.2, 3E1B10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67501-1-PBS targets RAB2A in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RAB2A fusion protein Ag7939 相同性解析による交差性が予測される生物種
Full Name RAB2A, member RAS oncogene family
Calculated molecular weight24 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC008929
Gene Symbol RAB2A
Gene ID (NCBI) 5862
RRIDAB_2882725
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61019
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize RAB2A and RAB2B.