Validation Data Gallery
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Tested Applications
| Positive WB detected in | human brain tissue, rat brain tissue, mouse kidney tissue, HeLa cells, mouse brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
11756-1-AP targets RAB2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RAB2B fusion protein Ag2352 相同性解析による交差性が予測される生物種 |
| Full Name | RAB2B, member RAS oncogene family |
| Calculated molecular weight | 216 aa, 24 kDa |
| Observed molecular weight | 24 kDa |
| GenBank accession number | BC020839 |
| Gene Symbol | RAB2B |
| Gene ID (NCBI) | 84932 |
| RRID | AB_2176900 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WUD1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize both RAB2A and RAB2B.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB2B antibody 11756-1-AP | Download protocol |
| IHC protocol for RAB2B antibody 11756-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion
| ||
Dis Markers Differential Plasma Proteins Identified via iTRAQ-Based Analysis Serve as Diagnostic Markers of Pancreatic Ductal Adenocarcinoma | ||
Dev Cell Setdb1 protects genome integrity in murine muscle stem cells to allow for regenerative myogenesis and inflammation |