Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 10999-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10999-1-AP (RAB32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 10999-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10999-1-AP (RAB32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 10999-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 10999-1-AP (RAB32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293T using 10999-1-AP
IP result of anti-RAB32 (IP:10999-1-AP, 4ug; Detection:10999-1-AP 1:500) with HEK-293T cells lysate 1560 ug.
IHC staining of human colon using 10999-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10999-1-AP (RAB32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 10999-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10999-1-AP (RAB32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 10999-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10999-1-AP (RAB32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 10999-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10999-1-AP (RAB32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U-251 using 10999-1-AP
Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RAB32 antibody (10999-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

RAB32 Polyclonal antibody

RAB32 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10999-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Ras-related protein Rab-32, Ras related protein Rab 32

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293T cells, HeLa cells, human colon tissue, HepG2 cells
Positive IP detected in	HEK-293T cells
Positive IHC detected in	human colon tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U-251 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 2 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

10999-1-AP targets RAB32 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RAB32 fusion protein Ag1450 相同性解析による交差性が予測される生物種
Full Name	RAB32, member RAS oncogene family
Calculated molecular weight	25 kDa
Observed molecular weight	25-28 kDa
GenBank accession number	BC015061
Gene Symbol	RAB32
Gene ID (NCBI)	10981
RRID	AB_2177047
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13637
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RAB32 antibody 10999-1-AP	Download protocol
IHC protocol for RAB32 antibody 10999-1-AP	Download protocol
IF protocol for RAB32 antibody 10999-1-AP	Download protocol
IP protocol for RAB32 antibody 10999-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Cell Death Dis Rab32 promotes glioblastoma migration and invasion via regulation of ERK/Drp1-mediated mitochondrial fission Authors - Pin Chen KD Validated View Article
mouse	WB,IF	mSphere Burkholderia pseudomallei BopE suppresses the Rab32-dependent defense pathway to promote its intracellular replication and virulence Authors - Chenglong Rao KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10999-1-AP

WB analysis using 10999-1-AP

WB analysis of human colon using 10999-1-AP

IP experiment of HEK-293T using 10999-1-AP

IHC staining of human colon using 10999-1-AP