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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB33A Polyclonal antibody

RAB33A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11025-1-AP

Synonyms

Small GTP-binding protein S10, Small GTP binding protein S10, Ras-related protein Rab-33A, Ras related protein Rab 33A, RABS10

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using RAB33A Polyclonal antibody (11025-1-AP)

    WB analysis of mouse brain using 11025-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11025-1-AP (RAB33A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using RAB33A Polyclonal antibody (11025-1-AP)

    WB analysis of human brain using 11025-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11025-1-AP (RAB33A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using RAB33A Polyclonal antibody (11025-1-AP)

    IHC staining of human brain using 11025-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11025-1-AP (RAB33A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using RAB33A Polyclonal antibody (11025-1-AP)

    IHC staining of human brain using 11025-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11025-1-AP (RAB33A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using RAB33A Polyclonal antibody (11025-1-AP)

    IHC staining of human lymphoma using 11025-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11025-1-AP (RAB33A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RAB33A Polyclonal antibody (11025-1-AP)

    IHC staining of human gliomas using 11025-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11025-1-AP (RAB33A antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using RAB33A Polyclonal antibody (11025-1-AP)

    IF Staining of PC-12 using 11025-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using RAB33A antibody (11025-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue
Positive IHC detected inhuman brain tissue, human gliomas tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11025-1-AP targets RAB33A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB33A fusion protein Ag1492 相同性解析による交差性が予測される生物種
Full Name RAB33A, member RAS oncogene family
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC009996
Gene symbol RAB33A
Gene ID (NCBI) 9363
RRIDAB_2238178
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAB33A antibody 11025-1-APDownload protocol
IHC protocol for RAB33A antibody 11025-1-APDownload protocol
IF protocol for RAB33A antibody 11025-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB, IF

Biochem Biophys Res Commun

The small GTPase Rab33A participates in regulation of amylase release from parotid acinar cells.

Authors - Akane Imai
View Article