Validation Data Gallery
Tested Applications
| Positive WB detected in | pig brain tissue, SH-SY5Y cells, rabbit brain tissue, rat brain tissue, mouse brain tissue |
| Positive IF/ICC detected in | Neuro-2a cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
68389-1-Ig targets RAB33A in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse, pig, rabbit samples.
| Tested Reactivity | Human, rat, mouse, pig, rabbit |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RAB33A fusion protein Ag33188 相同性解析による交差性が予測される生物種 |
| Full Name | RAB33A, member RAS oncogene family |
| Calculated molecular weight | 27 kDa |
| GenBank accession number | BC009996 |
| Gene Symbol | RAB33A |
| Gene ID (NCBI) | 9363 |
| RRID | AB_3085107 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q14088 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ras-related protein Rab-33A (RAB33A) is also named as RABS10, and belongs to the small GTPase superfamily and the Rab family. RAB33A is an X-linked gene that is expressed in brain, lymphocytes, and normal melanocytes, but is downregulated in melanoma cells. In the brain, RAB33A is present throughout the cortex, as well as in the hippocampal CA fields (PMID:16810302). RAB33A is expressed in cells, including neurons, lymphocytes, melanocytes and parotid acinar cells (PMID:16810302, PMID:25871792). In cultured rat hippocampal neurons, RAB33A is localized to the Golgi apparatus and post-Golgi vesicles transported along axons (PMID:22972995).In addition, RAB33A interacts with singar1/RUFY3, which suppresses the formation of surplus axons in cultured hippocampal neurons (PMID:21737958, PMID:17439943).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB33A antibody 68389-1-Ig | Download protocol |
| IF protocol for RAB33A antibody 68389-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Neurosci Rab6-Mediated Polarized Transport of Synaptic Vesicle Precursors Is Essential for the Establishment of Neuronal Polarity and Brain Formation |