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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB33B Polyclonal antibody

RAB33B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27349-1-AP

Synonyms

Ras-related protein Rab-33B, Ras related protein Rab 33B

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RAB33B Polyclonal antibody (27349-1-AP)

    WB analysis of HeLa using 27349-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RAB33B Polyclonal antibody (27349-1-AP)

    WB analysis using 27349-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RAB33B Polyclonal antibody (27349-1-AP)

    WB analysis of MCF-7 using 27349-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RAB33B Polyclonal antibody (27349-1-AP)

    WB analysis of HEK-293 using 27349-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RAB33B Polyclonal antibody (27349-1-AP)

    IHC staining of human stomach using 27349-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27349-1-AP (RAB33B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using RAB33B Polyclonal antibody (27349-1-AP)

    IHC staining of human stomach using 27349-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27349-1-AP (RAB33B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, U-87 MG cells, HEK-293 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27349-1-AP targets RAB33B in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB33B fusion protein Ag24181 相同性解析による交差性が予測される生物種
Full Name RAB33B, member RAS oncogene family
Calculated molecular weight 229 aa, 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC036064
Gene symbol RAB33B
Gene ID (NCBI) 83452
RRIDAB_2880851
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB33B is a small GTP-binding protein of the Rab GTPase family, whose members function in vesicle transport during protein secretion and endocytosis. Rab GTPases are active, membrane-associated proteins that recruit effector proteins in the GTP-bound state and inactive cytosolic proteins when in a GDP-bound state. RAB33B is ubiquitously expressed and has been implicated in Golgi to endoplasmic reticulum cycling of Golgi enzymes. In addition, RAB33B regulates Golgi homeostasis and coordinates intra-Golgi retrograde trafficking.

Protocols

Product Specific Protocols
WB protocol for RAB33B antibody 27349-1-APDownload protocol
IHC protocol for RAB33B antibody 27349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Pathog

Legionella hijacks the host Golgi-to-ER retrograde pathway for the association of Legionella-containing vacuole with the ER.

Authors - Mio Kawabata
View Article