- Featured Product
- KD/KO Validated
RAB34 Polyclonal antibody
RAB34 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 27435-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, U2OS cells, A549 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IF | See 5 publications below |
Product Information
27435-1-AP targets RAB34 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RAB34 fusion protein Ag26696 相同性解析による交差性が予測される生物種 |
| Full Name | RAB34, member RAS oncogene family |
| Calculated molecular weight | 251 aa, 28 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC016841 |
| Gene symbol | RAB34 |
| Gene ID (NCBI) | 83871 |
| RRID | AB_2880870 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RAB34, a Rab protein family member, is involved in repositioning of lysosomes, activation of micropinocytosis, and protein transport. RAB34 is aberrantly expressed in various cancers and exhibits oncogenic properties. Recently, Rab34 is identified as a ciliary protein. Rab34 is required for internal assembly of cilia but not for cilia built on the cell surface.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB34 antibody 27435-1-AP | Download protocol |
| IHC protocol for RAB34 antibody 27435-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Curr Biol Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway.
| ||
Biomedicines Signaling Switching from Hedgehog-GLI to MAPK Signaling Potentially Serves as a Compensatory Mechanism in Melanoma Cell Lines Resistant to GANT-61 | ||
Hum Mol Genet Pathogenic RAB34 variants impair primary cilium assembly and cause a novel oral-facial-digital syndrome | ||
bioRxiv A hierarchical pathway for assembly of the distal appendages that organize primary cilia | ||
bioRxiv Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages |
