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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

RAB39 Polyclonal antibody

RAB39 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13355-1-AP

Synonyms

Rab 39, RAB39, RAB39A, Ras related protein Rab 39A

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using RAB39 Polyclonal antibody (13355-1-AP)

    WB analysis of mouse brain using 13355-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13355-1-AP (RAB39 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using RAB39 Polyclonal antibody (13355-1-AP)

    WB analysis of human brain using 13355-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13355-1-AP (RAB39 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using RAB39 Polyclonal antibody (13355-1-AP)

    IP experiment of mouse brain using 13355-1-AP

    IP result of anti-RAB39 (IP:13355-1-AP, 4ug; Detection:13355-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RAB39 Polyclonal antibody (13355-1-AP)

    IHC staining of human gliomas using 13355-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13355-1-AP (RAB39 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RAB39 Polyclonal antibody (13355-1-AP)

    IHC staining of human gliomas using 13355-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13355-1-AP (RAB39 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using RAB39 Polyclonal antibody (13355-1-AP)

    IHC staining of human brain using 13355-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13355-1-AP (RAB39 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RAB39 Polyclonal antibody (13355-1-AP)

    IHC staining of human brain using 13355-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13355-1-AP (RAB39 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RAB39 Polyclonal antibody (13355-1-AP)

    IHC staining of human gliomas using 13355-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 13355-1-AP (RAB39 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RAB39 Polyclonal antibody (13355-1-AP)

    IHC staining of human gliomas using 13355-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 13355-1-AP (RAB39 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RAB39 Polyclonal antibody (13355-1-AP)

    IHC staining of human brain using 13355-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 13355-1-AP (RAB39 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RAB39 Polyclonal antibody (13355-1-AP)

    IHC staining of human brain using 13355-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 13355-1-AP (RAB39 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13355-1-AP targets RAB39 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB39 fusion protein Ag4151 相同性解析による交差性が予測される生物種
Full Name RAB39, member RAS oncogene family
Calculated molecular weight 217 aa, 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC028064
Gene symbol RAB39
Gene ID (NCBI) 54734
RRIDAB_2284908
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAB39 antibody 13355-1-APDownload protocol
IHC protocol for RAB39 antibody 13355-1-APDownload protocol
IP protocol for RAB39 antibody 13355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy

Authors - Shen Zhang
  • KD Validated
View Article
humanWB

Oncotarget

Prominent role of RAB39A-RXRB axis in cancer development and stemness.

Authors - Tokuhiro Chano
View Article
humanIF

J Biochem

RAB39A: a Rab small GTPase with a prominent role in cancer stemness.

Authors - Tokuhiro Chano
View Article
canineWB,IP

Cell Rep

Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.

Authors - Takahide Matsui
View Article