Validation Data Gallery
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Tested Applications
| Positive WB detected in | fetal human brain tissue, mouse brain tissue, Y79 cells, human brain tissue |
| Positive IP detected in | SH-SY5Y cells, mouse brain tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 9 publications below |
| IF | See 4 publications below |
| IP | See 1 publications below |
Product Information
12162-1-AP targets RAB39B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RAB39B fusion protein Ag2803 相同性解析による交差性が予測される生物種 |
| Full Name | RAB39B, member RAS oncogene family |
| Calculated molecular weight | 213 aa, 25 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC009714 |
| Gene Symbol | RAB39B |
| Gene ID (NCBI) | 116442 |
| RRID | AB_2177360 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96DA2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB39B antibody 12162-1-AP | Download protocol |
| IHC protocol for RAB39B antibody 12162-1-AP | Download protocol |
| IP protocol for RAB39B antibody 12162-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy
| ||
Am J Hum Genet Mutations in RAB39B Cause X-Linked Intellectual Disability and Early-Onset Parkinson Disease with α-Synuclein Pathology. | ||
EMBO J Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death. | ||
Acta Neuropathol Commun Synaptic localization of C9orf72 regulates post-synaptic glutamate receptor 1 levels. | ||
Brain Pathol RAB39B is redistributed in dementia with Lewy bodies and is sequestered within aβ plaques and Lewy bodies. |