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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB3A-specific Polyclonal antibody

RAB3A-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16865-1-AP

Synonyms

RAB3A, Ras-related protein Rab-3A, Ras related protein Rab 3A

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using RAB3A-specific Polyclonal antibody (16865-1-AP)

    WB analysis of mouse brain using 16865-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16865-1-AP (RAB3A-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using RAB3A-specific Polyclonal antibody (16865-1-AP)

    WB analysis of rat brain using 16865-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 16865-1-AP (RAB3A-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using RAB3A-specific Polyclonal antibody (16865-1-AP)

    IHC staining of human pancreas using 16865-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16865-1-AP (RAB3A-specific Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using RAB3A-specific Polyclonal antibody (16865-1-AP)

    IHC staining of human pancreas using 16865-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16865-1-AP (RAB3A-specific Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB3A-specific Polyclonal antibody (16865-1-AP)

    IF Staining of HeLa using 16865-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB3A-specific antibody (16865-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16865-1-AP targets RAB3A-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name RAB3A, member RAS oncogene family
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberNM_002866
Gene symbol RAB3A
Gene ID (NCBI) 5864
RRIDAB_11182389
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Rab3 subfamily includes Rab3A, Rab3B, Rab3C, and Rab3D, involving in the exocytosis of neurotransmitters and hormones. Rab 3A is found to be abundant in presynaptic nerve terminals and is also found to be crucial in acrosomal exocytosis in human spermatozoa. This antibody is specific to Rab3A.

Protocols

Product Specific Protocols
WB protocol for RAB3A-specific antibody 16865-1-APDownload protocol
IHC protocol for RAB3A-specific antibody 16865-1-APDownload protocol
IF protocol for RAB3A-specific antibody 16865-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols