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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB3IL1 Polyclonal antibody

RAB3IL1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17827-1-AP

Synonyms

Rabin3-like 1, Rabin3 like 1, Rab3A-interacting-like protein 1, Rab-3A-interacting-like protein 1, Guanine nucleotide exchange factor for Rab-3A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB3IL1 Polyclonal antibody (17827-1-AP)

    WB analysis using 17827-1-AP

    Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 17827-1-AP (RAB3IL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using RAB3IL1 Polyclonal antibody (17827-1-AP)

    WB analysis of human testis using 17827-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 17827-1-AP (RAB3IL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB3IL1 Polyclonal antibody (17827-1-AP)

    IF Staining of HeLa using 17827-1-AP

    Immunofluorescent analysis of Hela cells, using RAB3IL1 antibody 17827-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected infetal human brain tissue, human testis tissue
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17827-1-AP targets RAB3IL1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB3IL1 fusion protein Ag12204 相同性解析による交差性が予測される生物種
Full Name RAB3A interacting protein (rabin3)-like 1
Calculated molecular weight 382 aa, 43 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC022239
Gene symbol RAB3IL1
Gene ID (NCBI) 5866
RRIDAB_2300814
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAB3IL1 antibody 17827-1-APDownload protocol
IF protocol for RAB3IL1 antibody 17827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Small GTPases

Guanine nucleotide exchange factors activate Rab8a for Toll-like receptor signalling.

Authors - Samuel J Tong
View Article
mouseIF

Development

Identification of a heterogeneous and dynamic ciliome during embryonic development and cell differentiation

Authors - Kelsey H Elliott
View Article