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  • Featured Product
  • KD/KO Validated

RAB3IP Polyclonal antibody, PBS Only

RAB3IP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12321-1-PBS

Synonyms

RAB3IP/Rabin8, Rabin8, Rabin 3, Rab3A-interacting protein, Rab-3A-interacting protein

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using RAB3IP Polyclonal antibody (12321-1-AP)

    WB analysis of human brain using 12321-1-AP (same clone as 12321-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12321-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RAB3IP Polyclonal antibody (12321-1-AP)

    WB analysis using 12321-1-AP (same clone as 12321-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12321-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using RAB3IP Polyclonal antibody (12321-1-AP)

    WB analysis of mouse heart using 12321-1-AP (same clone as 12321-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12321-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using RAB3IP Polyclonal antibody (12321-1-AP)

    WB analysis of mouse lung using 12321-1-AP (same clone as 12321-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12321-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using RAB3IP Polyclonal antibody (12321-1-AP)

    WB analysis of human heart using 12321-1-AP (same clone as 12321-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12321-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using RAB3IP Polyclonal antibody (12321-1-AP)

    IP experiment of mouse lung using 12321-1-AP (same clone as 12321-1-PBS)

    IP result of anti-RAB3IP (IP:12321-1-AP, 3ug; Detection:12321-1-AP 1:1000) with mouse lung tissue lysate 4500ug. This data was developed using the same antibody clone with 12321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RAB3IP Polyclonal antibody (12321-1-AP)

    IHC staining of human stomach cancer using 12321-1-AP (same clone as 12321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12321-1-AP (RAB3IP antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12321-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RAB3IP Polyclonal antibody (12321-1-AP)

    IF Staining of HepG2 using 12321-1-AP (same clone as 12321-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RAB3IP/Rabin8 antibody (12321-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12321-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12321-1-PBS targets RAB3IP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB3IP fusion protein Ag2974 相同性解析による交差性が予測される生物種
Full Name RAB3A interacting protein (rabin3)
Calculated molecular weight 476 aa, 53 kDa
Observed molecular weight 53/51/43 kDa
GenBank accession numberBC015548
Gene symbol RAB3IP
Gene ID (NCBI) 117177
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport,but not for exocytosis of discoidal/fusiform-shaped vesicles. This antibody can recognize isoforms with predicted MW's of 53 kDa, 51 kDa, 43 kDa, 45 kDa, 33 kDa and 35 kDa, 29 kDa respectively.