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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB3IP/Rabin8 Polyclonal antibody (29818-1-AP)

    WB analysis using 29818-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29818-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using RAB3IP/Rabin8 Polyclonal antibody (29818-1-AP)

    IP experiment of Jurkat using 29818-1-AP

    IP result of anti-RAB3IP/Rabin8 (IP:29818-1-AP, 4ug; Detection:29818-1-AP 1:4000) with Jurkat cells lysate 1320 ug.

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RAB3IP/Rabin8 Polyclonal antibody

RAB3IP/Rabin8 Polyclonal Antibody for WB, IP, ELISA
Cat No. 29818-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

RAB3IP, Rabin8, Rabin 3, Rab3A-interacting protein, Rab-3A-interacting protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29818-1-AP targets RAB3IP/Rabin8 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB3IP/Rabin8 fusion protein Ag31291 相同性解析による交差性が予測される生物種
Full Name RAB3A interacting protein (rabin3)
Calculated molecular weight 476 aa, 53 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC015548
Gene Symbol RAB3IP
Gene ID (NCBI) 117177
RRIDAB_3669697
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QF0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport,but not for exocytosis of discoidal/fusiform-shaped vesicles.

Protocols

Product Specific Protocols
WB protocol for RAB3IP/Rabin8 antibody 29818-1-APDownload protocol
IP protocol for RAB3IP/Rabin8 antibody 29818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols