Validation Data Gallery

Filter: AllWBIPIHCIF-P

WB analysis using 11947-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11947-1-AP (RAB5A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11947-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11947-1-AP (RAB5A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 11947-1-AP
IP result of anti-RAB5A (IP:11947-1-AP, 4ug; Detection:11947-1-AP 1:10000) with mouse brain tissue lysate 1680 ug.
IHC staining of human gliomas using 11947-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11947-1-AP (RAB5A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 11947-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11947-1-AP (RAB5A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11947-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11947-1-AP (RAB5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 11947-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11947-1-AP (RAB5A Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human pancreas using 11947-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11947-1-AP (RAB5A Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of mouse brain using 11947-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using RAB5A antibody (11947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 11947-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using RAB5A antibody (11947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

RAB5A Polyclonal antibody

RAB5A Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 11947-1-AP

Publications(57)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

RAB5, EC:3.6.5.2, Ras related protein Rab 5A, Ras-related protein Rab-5A

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (57)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, human brain tissue, mouse brain tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human gliomas tissue, mouse brain tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 9 publications below
WB	See 38 publications below
IHC	See 7 publications below
IF	See 25 publications below
IP	See 2 publications below

Product Information

11947-1-AP targets RAB5A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2549 Product name: Recombinant human RAB5A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-215 aa of BC001267 Sequence: MASRGATRPNGPNTGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASPNIVIALSGNKADLANKRAVDFQEAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN 相同性解析による交差性が予測される生物種
Full Name	RAB5A, member RAS oncogene family
Calculated molecular weight	215 aa, 24 kDa
Observed molecular weight	24 kDa
GenBank accession number	BC001267
Gene Symbol	RAB5A
Gene ID (NCBI)	5868
RRID	AB_2269388
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P20339
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for RAB5A antibody 11947-1-AP	Download protocol
IHC protocol for RAB5A antibody 11947-1-AP	Download protocol
IP protocol for RAB5A antibody 11947-1-AP	Download protocol
WB protocol for RAB5A antibody 11947-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cell Metab Sensing steroid hormone 17α-hydroxypregnenolone by GPR56 enables protection from ferroptosis-induced liver injury Authors - Hui Lin View Article
mouse	WB	Cell Metab Hepatic lipid remodeling in cold exposure uncovers direct regulation of bis(monoacylglycero)phosphate lipids by phospholipase A2 group XV Authors - Jessica W Davidson View Article
		Immunity trans-Endothelial neutrophil migration activates bactericidal function via Piezo1 mechanosensing Authors - Amitabha Mukhopadhyay View Article
human	WB	Immunity Targeting intracellular oncoproteins with dimeric IgA promotes expulsion from the cytoplasm and immune-mediated control of epithelial cancers Authors - Subir Biswas View Article
human	IF	Nat Genet Identification of TMEM230 mutations in familial Parkinson's disease. Authors - Han-Xiang Deng View Article
mouse	WB	Autophagy RAB7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging. Authors - Xin Jin View Article

Validation Data Gallery

WB analysis using 11947-1-AP

WB analysis of human brain using 11947-1-AP

IP experiment of mouse brain using 11947-1-AP

IHC staining of human gliomas using 11947-1-AP

IHC staining of human gliomas using 11947-1-AP

IHC staining of mouse brain using 11947-1-AP

IHC staining of human pancreas using 11947-1-AP

IHC staining of human pancreas using 11947-1-AP

IF Staining of mouse brain using 11947-1-AP

IF Staining of mouse brain using 11947-1-AP

RAB5A Polyclonal antibody

RAB5A Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Sensing steroid hormone 17α-hydroxypregnenolone by GPR56 enables protection from ferroptosis-induced liver injury

Hepatic lipid remodeling in cold exposure uncovers direct regulation of bis(monoacylglycero)phosphate lipids by phospholipase A2 group XV

trans-Endothelial neutrophil migration activates bactericidal function via Piezo1 mechanosensing

Targeting intracellular oncoproteins with dimeric IgA promotes expulsion from the cytoplasm and immune-mediated control of epithelial cancers

Identification of TMEM230 mutations in familial Parkinson's disease.

RAB7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging.

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