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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB5A-Specific Polyclonal antibody

RAB5A-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20228-1-AP

Synonyms

RAB5, RAB5A, RAB5A-Specific, Ras related protein Rab 5A

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using RAB5A-Specific Polyclonal antibody (20228-1-AP)

    WB analysis of human brain using 20228-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using RAB5A-Specific Polyclonal antibody (20228-1-AP)

    WB analysis of mouse colon using 20228-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using RAB5A-Specific Polyclonal antibody (20228-1-AP)

    WB analysis of SH-SY5Y using 20228-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using RAB5A-Specific Polyclonal antibody (20228-1-AP)

    WB analysis of human brain using 20228-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using RAB5A-Specific Polyclonal antibody (20228-1-AP)

    IP experiment of mouse brain using 20228-1-AP

    IP result of anti-RAB5A-Specific (IP:20228-1-AP, 4ug; Detection:20228-1-AP 1:600) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using RAB5A-Specific Polyclonal antibody (20228-1-AP)

    IHC staining of human brain using 20228-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20228-1-AP (RAB5A-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using RAB5A-Specific Polyclonal antibody (20228-1-AP)

    IHC staining of human brain using 20228-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20228-1-AP (RAB5A-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using RAB5A-Specific Polyclonal antibody (20228-1-AP)

    IHC staining of human brain using 20228-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20228-1-AP (RAB5A-Specific Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RAB5A-Specific Polyclonal antibody (20228-1-AP)

    IHC staining of human brain using 20228-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20228-1-AP (RAB5A-Specific Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RAB5A-Specific Polyclonal antibody (20228-1-AP)

    IHC staining of human placenta using 20228-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RAB5A-Specific Polyclonal antibody (20228-1-AP)

    IHC staining of human placenta using 20228-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inhuman brain tissue, mouse colon tissue, SH-SY5Y cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

20228-1-AP targets RAB5A-Specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name RAB5A, member RAS oncogene family
Calculated molecular weight 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberNM_004162
Gene symbol RAB5A
Gene ID (NCBI) 5868
RRIDAB_10694147
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB5A belongs to the small GTPase superfamily and Rab family. It is required for the fusion of plasma membranes and early endosomes. The antibody is specific to RAB5A. It has no cross reaction to RAB5B or RAB5C.

Protocols

Product Specific Protocols
WB protocol for RAB5A-Specific antibody 20228-1-APDownload protocol
IHC protocol for RAB5A-Specific antibody 20228-1-APDownload protocol
IP protocol for RAB5A-Specific antibody 20228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Front Physiol

Overexpression of ameloblastin in secretory ameloblasts results in demarcated, hypomineralized opacities in enamel

Authors - Yong-Hee Patricia Chun
View Article