Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 55469-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 55469-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 55469-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 55469-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 55469-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 55469-1-AP
IP result of anti-RAB7A (IP:55469-1-AP, 4ug; Detection:55469-1-AP 1:5000) with A431 cells lysate 720 ug.
IHC staining of human kidney using 55469-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55469-1-AP (RAB7A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 55469-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55469-1-AP (RAB7A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skeletal muscle using 55469-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 55469-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human stomach using 55469-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach using 55469-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of mouse spinal cord using 55469-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spinal cord tissue using RAB7A antibody (55469-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse spinal cord using 55469-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spinal cord tissue using RAB7A antibody (55469-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 55469-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAB7A antibody (55469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of A431 using 55469-1-AP
1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RAB7A (55469-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

RAB7A Polyclonal antibody

RAB7A Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 55469-1-AP

Publications(112)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Synonyms

RAB7, EC:3.6.5.2, Ras related protein Rab 7a, Ras-related protein Rab-7a

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®594

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (112)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	rat brain tissue, A431 cells, L-929 cells, NIH/3T3 cells, SH-SY5Y cells, RAW 264.7 cells, C6 cells, mouse brain tissue
Positive IP detected in	A431 cells
Positive IHC detected in	human kidney tissue, human skeletal muscle tissue, human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse spinal cord tissue
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 71 publications below
IHC	See 4 publications below
IF	See 11 publications below
IP	See 4 publications below
FC	See 1 publications below
CoIP	See 2 publications below

Product Information

55469-1-AP targets RAB7A in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, zebrafish, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	RAB7A, member RAS oncogene family
Calculated molecular weight	23 kDa
Observed molecular weight	23 kDa
GenBank accession number	NM_004637
Gene Symbol	RAB7A
Gene ID (NCBI)	7879
RRID	AB_11182831
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P51149
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.

Protocols

Product Specific Protocols
FC protocol for RAB7A antibody 55469-1-AP	Download protocol
IF protocol for RAB7A antibody 55469-1-AP	Download protocol
IHC protocol for RAB7A antibody 55469-1-AP	Download protocol
IP protocol for RAB7A antibody 55469-1-AP	Download protocol
WB protocol for RAB7A antibody 55469-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1. Authors - Wenchang Xiao View Article
		Cell Metab Oral GLP-1 receptor agonist promotes astrocyte-neuron lactate and lipid transfer with neuroprotective effects. Authors - Yixuan Du View Article
human	WB	Cell Res RAB31 marks and controls an ESCRT-independent exosome pathway. Authors - Denghui Wei View Article
human	WB	Immunity Targeting intracellular oncoproteins with dimeric IgA promotes expulsion from the cytoplasm and immune-mediated control of epithelial cancers Authors - Subir Biswas View Article
human	WB	Nat Biomed Eng A deep learning and large language hybrid workflow for omics interpretation. Authors - Dachao Tang View Article
human	WB	Nat Cell Biol DNA nanodevices detect an acidic nanolayer on the lysosomal surface. Authors - Yutong Zhang View Article

Validation Data Gallery

WB analysis using 55469-1-AP

WB analysis using 55469-1-AP

WB analysis of NIH/3T3 using 55469-1-AP

WB analysis of SH-SY5Y using 55469-1-AP

WB analysis of A431 using 55469-1-AP

IP experiment of A431 using 55469-1-AP

IHC staining of human kidney using 55469-1-AP

IHC staining of human kidney using 55469-1-AP

IHC staining of human skeletal muscle using 55469-1-AP

IHC staining of human skeletal muscle using 55469-1-AP

IHC staining of human stomach using 55469-1-AP

IHC staining of human stomach using 55469-1-AP

IF Staining of mouse spinal cord using 55469-1-AP

IF Staining of mouse spinal cord using 55469-1-AP

IF Staining of HeLa using 55469-1-AP

FC experiment of A431 using 55469-1-AP

RAB7A Polyclonal antibody

RAB7A Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1.

Oral GLP-1 receptor agonist promotes astrocyte-neuron lactate and lipid transfer with neuroprotective effects.

RAB31 marks and controls an ESCRT-independent exosome pathway.

Targeting intracellular oncoproteins with dimeric IgA promotes expulsion from the cytoplasm and immune-mediated control of epithelial cancers

A deep learning and large language hybrid workflow for omics interpretation.

DNA nanodevices detect an acidic nanolayer on the lysosomal surface.

Able™

Able^™