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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB8A Polyclonal antibody (30636-1-AP)

    WB analysis using 30636-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30636-1-AP (RAB8A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using RAB8A Polyclonal antibody (30636-1-AP)

    IF Staining of MDCK using 30636-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using RAB8A antibody (30636-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RAB8A Polyclonal antibody (30636-1-AP)

    IF Staining of NIH/3T3 using 30636-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using RAB8A antibody (30636-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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RAB8A Polyclonal antibody

RAB8A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30636-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, canine, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

MEL, Oncogene c mel, RAB8, RAB8A, Ras related protein Rab 8A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inNIH/3T3 cells, MDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30636-1-AP targets RAB8A in WB, IF/ICC, ELISA applications and shows reactivity with Human, canine, mouse samples.

Tested Reactivity Human, canine, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB8A fusion protein Ag30567 相同性解析による交差性が予測される生物種
Full Name RAB8A, member RAS oncogene family
Calculated molecular weight 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberNM_005370
Gene Symbol RAB8A
Gene ID (NCBI) 4218
RRIDAB_3086378
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61006
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B.

Protocols

Product Specific Protocols
WB protocol for RAB8A antibody 30636-1-APDownload protocol
IF protocol for RAB8A antibody 30636-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols