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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB8B Polyclonal antibody, PBS Only

RAB8B Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55295-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse skin tissue using RAB8B Polyclonal antibody (55295-1-AP)

    WB analysis of mouse skin using 55295-1-AP (same clone as 55295-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55295-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RAB8B Polyclonal antibody (55295-1-AP)

    WB analysis using 55295-1-AP (same clone as 55295-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55295-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using RAB8B Polyclonal antibody (55295-1-AP)

    WB analysis of human brain using 55295-1-AP (same clone as 55295-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55295-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using RAB8B Polyclonal antibody (55295-1-AP)

    WB analysis of mouse colon using 55295-1-AP (same clone as 55295-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55295-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skin tissue using RAB8B Polyclonal antibody (55295-1-AP)

    WB analysis of mouse skin using 55295-1-AP (same clone as 55295-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55295-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55295-1-PBS targets RAB8B in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name RAB8B, member RAS oncogene family
Calculated molecular weight 24 kDa
Observed molecular weight 28 kDa
GenBank accession numberNM_016530
Gene symbol RAB8B
Gene ID (NCBI) 51762
RRIDAB_10896304
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B. 55295-1-AP is specific to RAB8B and 55296-1-AP is specific to RAB8A.