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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB9A Recombinant antibody (84129-5-RR)

    WB analysis using 84129-5-RR (same clone as 84129-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84129-5-RR (RAB9A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84129-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using RAB9A Recombinant antibody (84129-5-RR)

    FC experiment of U2OS using 84129-5-RR (same clone as 84129-5-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug RAB9A Recombinant antibody (84129-5-RR, Clone:241372F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84129-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84129-5-RR

    Affinity and Kinetic Characterization of 84129-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84129-5-RR against Human RAB9A were performed. The affinity constant is 11.9 nM.

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RAB9A Recombinant antibody, PBS Only

RAB9A Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84129-5-PBS
Clone No.241372F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

RAB9, Ras-related protein Rab-9A, Ras related protein Rab 9A, 241372F8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84129-5-PBS targets RAB9A in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAB9A fusion protein Ag1969 相同性解析による交差性が予測される生物種
Full Name RAB9A, member RAS oncogene family
Calculated molecular weight 201 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC017265
Gene Symbol RAB9A
Gene ID (NCBI) 9367
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP51151
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RAB9A belongs to the small GTPase superfamily, the members of which play an important role in regulating vesicular trafficking and reside in specific intracellular compartments. RAB9A has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors, such as mannose 6-phosphate receptor, from early endosomes to the trans Golgi network.