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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB9A-Specific Polyclonal antibody

RAB9A-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18719-1-AP

Synonyms

RAB9, RAB9A, RAB9A-Specific, Ras related protein Rab 9A

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RAB9A-Specific Polyclonal antibody (18719-1-AP)

    WB analysis of HEK-293 using 18719-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18719-1-AP (RAB9A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RAB9A-Specific Polyclonal antibody (18719-1-AP)

    WB analysis of HeLa using 18719-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18719-1-AP (RAB9A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RAB9A-Specific Polyclonal antibody (18719-1-AP)

    IHC staining of human gliomas using 18719-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18719-1-AP (RAB9A-Specific Antibody) at dilution of 1:50.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18719-1-AP targets RAB9A-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name RAB9A, member RAS oncogene family
Calculated molecular weight 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC017265
Gene symbol RAB9A
Gene ID (NCBI) 9367
RRIDAB_2175604
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB9A, also named as RAB9, is a member of the Ras superfamily of small Rab GTPases. It is located in late endosomes. Rab9A interacts with its effector proteins p40 and TIP47 to promote the MPR (mannose 6-phosphate receptor)-associated lysosomal enzyme transport between late endosomes and the trans Golgi network. The antibody is specific to RAB9A.

Protocols

Product Specific Protocols
WB protocol for RAB9A-Specific antibody 18719-1-APDownload protocol
IHC protocol for RAB9A-Specific antibody 18719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols