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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using RAB9B Polyclonal antibody (18152-1-AP)

    WB analysis of HepG2 using 18152-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18152-1-AP (RAB9B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RAB9B Polyclonal antibody (18152-1-AP)

    WB analysis of HeLa using 18152-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18152-1-AP (RAB9B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RAB9B Polyclonal antibody (18152-1-AP)

    WB analysis of mouse brain using 18152-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18152-1-AP (RAB9B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB9B Polyclonal antibody (18152-1-AP)

    IF Staining of HeLa using 18152-1-AP

    IF result of RAB9B antibody (18152-1-AP, 1:200) detected with PFA fixed HeLa cells. Greem: RAB9B antibody stains late endosomes. Red, Golgi stain.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RAB9B Polyclonal antibody (18152-1-AP)

    IF Staining of HepG2 using 18152-1-AP

    Immunofluorescent analysis of HepG2 cells, using RAB9B antibody 18152-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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RAB9B Polyclonal antibody

RAB9B Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 18152-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Rab 9 like protein, Rab 9L, RAB9B, RAB9L, Ras related protein Rab 9B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 200 μg/ml
20 μL, 200 μg/ml
150 μL, 200 μg/ml

¥67,000/ 150 μL

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, HepG2 cells
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18152-1-AP targets RAB9B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB9B fusion protein Ag12848 相同性解析による交差性が予測される生物種
Full Name RAB9B, member RAS oncogene family
Calculated molecular weight 201 aa, 23 kDa
Observed molecular weight 23-25 kDa
GenBank accession numberBC093756
Gene Symbol RAB9B
Gene ID (NCBI) 51209
RRIDAB_10642146
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP90
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAB9B antibody 18152-1-APDownload protocol
IF protocol for RAB9B antibody 18152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols