Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, human liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
14350-1-AP targets RABEP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RABEP1 fusion protein Ag5693 相同性解析による交差性が予測される生物種 |
| Full Name | rabaptin, RAB GTPase binding effector protein 1 |
| Calculated molecular weight | 99 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC041700 |
| Gene Symbol | RABEP1 |
| Gene ID (NCBI) | 9135 |
| RRID | AB_2175644 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15276 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Rab GTPase-binding effector protein 1 is an enzyme that in humans is encoded by the RABEP1 gene. It belongs to rabaptin protein family. RABEP1 act as linker between gamma-adaptin, RAB4A and RAB5A. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. (PMID: 22841712)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RABEP1 antibody 14350-1-AP | Download protocol |
| IF protocol for RABEP1 antibody 14350-1-AP | Download protocol |
| IP protocol for RABEP1 antibody 14350-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors. | ||
J Cell Sci His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation
| ||
J Mol Cell Cardiol Decreased inspired oxygen stimulates de novo formation of coronary collaterals in adult heart. |