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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RABEP1 Monoclonal antibody, PBS Only

RABEP1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E7E11

Cat no : 68285-1-PBS

Synonyms

RAB5EP, Rabaptin 4, Rabaptin 5, Rabaptin 5alpha, RABEP1, RABPT5, RABPT5A

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RABEP1 Monoclonal antibody (68285-1-Ig)

    WB analysis using 68285-1-Ig (same clone as 68285-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68285-1-Ig (RABEP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68285-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RABEP1 Monoclonal antibody (68285-1-Ig)

    IF Staining of HeLa using 68285-1-Ig (same clone as 68285-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RABEP1 antibody (68285-1-Ig, Clone: 1E7E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68285-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RABEP1 Monoclonal antibody (68285-1-Ig)

    IF Staining of HeLa using 68285-1-Ig (same clone as 68285-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RABEP1 antibody (68285-1-Ig, Clone: 1E7E11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68285-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68285-1-PBS targets RABEP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RABEP1 fusion protein Ag6023 相同性解析による交差性が予測される生物種
Full Name rabaptin, RAB GTPase binding effector protein 1
Calculated molecular weight 99 kDa
Observed molecular weight100-105 kDa
GenBank accession numberBC041700
Gene symbol RABEP1
Gene ID (NCBI) 9135
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Rab GTPase-binding effector protein 1 is an enzyme that in humans is encoded by the RABEP1 gene. It belongs to rabaptin protein family. RABEP1 act as linker between gamma-adaptin, RAB4A and RAB5A. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. (PMID: 22841712)