Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human cervical cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IF | See 2 publications below |
Product Information
14625-1-AP targets RABEP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RABEP2 fusion protein Ag6197 相同性解析による交差性が予測される生物種 |
| Full Name | rabaptin, RAB GTPase binding effector protein 2 |
| Calculated molecular weight | 64 kDa |
| Observed molecular weight | 59-62 kDa |
| GenBank accession number | BC058900 |
| Gene Symbol | RABEP2 |
| Gene ID (NCBI) | 79874 |
| RRID | AB_2175655 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H5N1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RABEP2, also named as RABPT5B, plays a role in membrane trafficking and in homotypic early endosome fusion. It has two isoforms with MW 59-63 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RABEP2 antibody 14625-1-AP | Download protocol |
| IHC protocol for RABEP2 antibody 14625-1-AP | Download protocol |
| IF protocol for RABEP2 antibody 14625-1-AP | Download protocol |
| IP protocol for RABEP2 antibody 14625-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Am J Hum Genet A cross-species approach using an in vivo evaluation platform in mice demonstrates that sequence variation in human RABEP2 modulates ischemic stroke outcomes | ||
Stroke Variants of Rab GTPase-Effector Binding Protein-2 Cause Variation in the Collateral Circulation and Severity of Stroke. | ||
Sci Rep Rab-GTPase binding effector protein 2 (RABEP2) is a primed substrate for Glycogen Synthase kinase-3 (GSK3). | ||
J Mol Cell Cardiol Decreased inspired oxygen stimulates de novo formation of coronary collaterals in adult heart. | ||
J Hematol Oncol LncRNAs H19 and HULC, activated by oxidative stress, promote cell migration and invasion in cholangiocarcinoma through a ceRNA manner. | ||
Arterioscler Thromb Vasc Biol Hypoxia-Preconditioned Bone Marrow Mesenchymal Stem Cells Improved Cerebral Collateral Circulation and Stroke Outcome in Mice |