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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using RABEP2 Monoclonal antibody (60125-1-Ig)

    WB analysis of Jurkat using 60125-1-Ig (same clone as 60125-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60125-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using RABEP2 Monoclonal antibody (60125-1-Ig)

    WB analysis of HSC-T6 using 60125-1-Ig (same clone as 60125-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60125-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using RABEP2 Monoclonal antibody (60125-1-Ig)

    WB analysis of PC-3 using 60125-1-Ig (same clone as 60125-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60125-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RABEP2 Monoclonal antibody (60125-1-Ig)

    IHC staining of human prostate cancer using 60125-1-Ig (same clone as 60125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60125-1-Ig (RABEP2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60125-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RABEP2 Monoclonal antibody (60125-1-Ig)

    IHC staining of human prostate cancer using 60125-1-Ig (same clone as 60125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60125-1-Ig (RABEP2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60125-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RABEP2 Monoclonal antibody (60125-1-Ig)

    IHC staining of human breast cancer using 60125-1-Ig (same clone as 60125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60125-1-Ig (RABEP2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60125-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RABEP2 Monoclonal antibody (60125-1-Ig)

    IHC staining of human breast cancer using 60125-1-Ig (same clone as 60125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60125-1-Ig (RABEP2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60125-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

RABEP2 Monoclonal antibody, PBS Only

RABEP2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60125-1-PBS
Clone No.1A4A4

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

FRA, Rabaptin 5beta, RABEP2, RABPT5B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60125-1-PBS targets RABEP2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RABEP2 fusion protein Ag6197 相同性解析による交差性が予測される生物種
Full Name rabaptin, RAB GTPase binding effector protein 2
Calculated molecular weight 64 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC058900
Gene Symbol RABEP2
Gene ID (NCBI) 79874
RRIDAB_2284821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H5N1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RABEP2, also named as RABPT5B, plays a role in membrane trafficking and in homotypic early endosome fusion. It has two isoforms with MW 59-63 kDa.