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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RABEPK/p40 Polyclonal antibody

RABEPK/p40 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10213-2-AP

Synonyms

40 kDa Rab9 effector protein, p40, RAB9P40, RABEPK, RABEPK/p40

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using RABEPK/p40 Polyclonal antibody (10213-2-AP)

    WB analysis of HepG2 using 10213-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10213-2-AP (RABEPK/p40 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using RABEPK/p40 Polyclonal antibody (10213-2-AP)

    WB analysis of K-562 using 10213-2-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10213-2-AP (RABEPK/p40 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RABEPK/p40 Polyclonal antibody (10213-2-AP)

    WB analysis of HeLa using 10213-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10213-2-AP (RABEPK/p40 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RABEPK/p40 Polyclonal antibody (10213-2-AP)

    IHC staining of human lung cancer using 10213-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10213-2-AP (RABEPK/p40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RABEPK/p40 Polyclonal antibody (10213-2-AP)

    IHC staining of human lung cancer using 10213-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10213-2-AP (RABEPK/p40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10213-2-AP targets RABEPK/p40 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RABEPK/p40 fusion protein Ag0285 相同性解析による交差性が予測される生物種
Full Name Rab9 effector protein with kelch motifs
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC000503
Gene symbol RABEPK/p40
Gene ID (NCBI) 10244
RRIDAB_2238057
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.

Protocols

Product Specific Protocols
WB protocol for RABEPK/p40 antibody 10213-2-APDownload protocol
IHC protocol for RABEPK/p40 antibody 10213-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols