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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RABEPK/p40 Monoclonal antibody

RABEPK/p40 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E11A3

Cat no : 66622-1-Ig

Synonyms

p40, RABEPK, RAB9P40, Rab9 effector protein with kelch motifs, 40 kDa Rab9 effector protein

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using RABEPK/p40 Monoclonal antibody (66622-1-Ig)

    WB analysis of Jurkat using 66622-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using RABEPK/p40 Monoclonal antibody (66622-1-Ig)

    WB analysis of HSC-T6 using 66622-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using RABEPK/p40 Monoclonal antibody (66622-1-Ig)

    WB analysis of RAW 264.7 using 66622-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RABEPK/p40 Monoclonal antibody (66622-1-Ig)

    WB analysis of HeLa using 66622-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RABEPK/p40 Monoclonal antibody (66622-1-Ig)

    WB analysis of HEK-293 using 66622-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RABEPK/p40 Monoclonal antibody (66622-1-Ig)

    IHC staining of human lung cancer using 66622-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RABEPK/p40 Monoclonal antibody (66622-1-Ig)

    IHC staining of human lung cancer using 66622-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using RABEPK/p40 Monoclonal antibody (66622-1-Ig)

    IHC staining of human colon using 66622-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using RABEPK/p40 Monoclonal antibody (66622-1-Ig)

    IHC staining of human colon using 66622-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RABEPK/p40 Monoclonal antibody (66622-1-Ig)

    IF Staining of HeLa using 66622-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RABEPK/p40 antibody (66622-1-Ig, Clone: 1E11A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RABEPK/p40 Monoclonal antibody (66622-1-Ig)

    IF Staining of A549 using 66622-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RABEPK/p40 antibody (66622-1-Ig, Clone: 1E11A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HEK-293 cells, HSCT6 cells, RAW 264.7 cells
Positive IHC detected inhuman lung cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66622-1-Ig targets RABEPK/p40 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RABEPK/p40 fusion protein Ag7796 相同性解析による交差性が予測される生物種
Full Name Rab9 effector protein with kelch motifs
Calculated molecular weight 41 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC065725
Gene symbol RABEPK/p40
Gene ID (NCBI) 10244
RRIDAB_2881982
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.

Protocols

Product Specific Protocols
WB protocol for RABEPK/p40 antibody 66622-1-IgDownload protocol
IHC protocol for RABEPK/p40 antibody 66622-1-IgDownload protocol
IF protocol for RABEPK/p40 antibody 66622-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Exp Ther Med

Management of a rare ovarian carcinosarcoma: A case report and literature review

Authors - Jun Fu
View Article
ratWB,IF

J Med Virol

Borna disease virus 1 induces ferroptosis, contributing to lethal encephalitis

Authors - Qing Tan
View Article