Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human hepatocirrhosis tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
13894-1-AP targets RABGAP1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RABGAP1L fusion protein Ag4856 相同性解析による交差性が予測される生物種 |
| Full Name | RAB GTPase activating protein 1-like |
| Calculated molecular weight | 815 aa, 93 kDa |
| Observed molecular weight | 93-105 kDa |
| GenBank accession number | BC041888 |
| Gene Symbol | RABGAP1L |
| Gene ID (NCBI) | 9910 |
| RRID | AB_10638308 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5R372 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RABGAP1L, also known as TBC1D18, is a member of the Tre2/Bub2/Cdc16 (TBC)-domain-containing protein family. Overexpression of RABGAP1L potentiated the antiviral action of IFN against a panel of viruses, including IAV, and its function required a catalytically active TBC domain.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RABGAP1L antibody 13894-1-AP | Download protocol |
| IHC protocol for RABGAP1L antibody 13894-1-AP | Download protocol |
| IF protocol for RABGAP1L antibody 13894-1-AP | Download protocol |
| IP protocol for RABGAP1L antibody 13894-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer. | ||
Cell Rep Restriction factor screening identifies RABGAP1L-mediated disruption of endocytosis as a host antiviral defense. | ||
J Cell Biol TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1
| ||
BMC Pregnancy Childbirth Core biomarkers analysis benefit for diagnosis on human intrahepatic cholestasis of pregnancy |