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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RABGAP1L Recombinant antibody

RABGAP1L Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240663G12

Cat no : 83698-4-RR

Synonyms

Rab GTPase-activating protein 1-like, isoform 10, 240663G12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RABGAP1L Recombinant antibody (83698-4-RR)

    WB analysis using 83698-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83698-4-RR (RABGAP1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using RABGAP1L Recombinant antibody (83698-4-RR)

    FC experiment of HeLa using 83698-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RABGAP1L Recombinant antibody (83698-4-RR, Clone:240663G12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83698-4-RR

    Affinity and Kinetic Characterization of 83698-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83698-4-RR against Human RABGAP1L were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inK-562 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83698-4-RR is RABGAP1L Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RABGAP1L fusion protein Ag4856 相同性解析による交差性が予測される生物種
Full Name RAB GTPase activating protein 1-like
Calculated molecular weight 815 aa, 93 kDa
Observed molecular weight100 kDa,140 kDa
GenBank accession numberBC041888
Gene symbol RABGAP1L
Gene ID (NCBI) 9910
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RABGAP1L, also known as TBC1D18, is a member of the Tre2/Bub2/Cdc16 (TBC)-domain-containing protein family. Overexpression of RABGAP1L potentiated the antiviral action of IFN against a panel of viruses, including IAV, and its function required a catalytically active TBC domain.

Protocols

Product Specific Protocols
WB protocol for RABGAP1L antibody 83698-4-RRDownload protocol
FC protocol for RABGAP1L antibody 83698-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols