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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RABIF Polyclonal antibody

RABIF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29113-1-AP

Synonyms

MSS4, RAB interacting factor, RABIF, RASGFR3, RASGRF3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RABIF Polyclonal antibody (29113-1-AP)

    WB analysis using 29113-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29113-1-AP (RABIF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RABIF Polyclonal antibody (29113-1-AP)

    IHC staining of mouse brain using 29113-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29113-1-AP (RABIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RABIF Polyclonal antibody (29113-1-AP)

    IHC staining of human breast cancer using 29113-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29113-1-AP (RABIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RABIF Polyclonal antibody (29113-1-AP)

    IF Staining of MCF-7 using 29113-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RABIF antibody (29113-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, Jurkat cells
Positive IHC detected inmouse brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29113-1-AP targets RABIF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RABIF fusion protein Ag30761 相同性解析による交差性が予測される生物種
Full Name RAB interacting factor
Calculated molecular weight 123 aa, 14 kDa
Observed molecular weight14 kDa
GenBank accession numberBC018488
Gene symbol RABIF
Gene ID (NCBI) 5877
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RABIF (Rab-interacting factor)/MSS4 (mammalian suppressor of Sec4) was the first putative guanine nucleotide exchange factors (GEFs) isolated for Rabs, which may act as a chaperone, allowing interacting Rab proteins to be properly activated where and when they are needed in the cell (PMID: 25158853). RABIF is a 17-kDa protein that is expressed in all mammalian tissues and is in complex with nucleotide-free Rab8 GTPase and Rab10 GTPase (PMID: 28894007, 28228250).

Protocols

Product Specific Protocols
WB protocol for RABIF antibody 29113-1-APDownload protocol
IHC protocol for RABIF antibody 29113-1-APDownload protocol
IF protocol for RABIF antibody 29113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols